Recombinant CD35 antibody

Catalog No. ABIN7072539

This anti-CD35 antibody is a Rabbit Chimeric antibody detecting CD35 in WB, IF and ELISA. Suitable for Human.

Quick Overview for Recombinant CD35 antibody (ABIN7072539)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD35 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Clone: E11

Product Details anti-CD35 Antibody

Purpose: Anti-CR1 [E11], Rabbit IgG, kappa

Specificity: E11 is reactive to neutrophilic granulocytes and eosinophils, monocytes, macrophages, lymphocytes, erythrocytes, dendritic reticulum cells, tonsil tissue section, spleen tissue section, lymph node tissue section, gut tissue section, kidney podocytes and kidney tissue sections, chronic lymphocytic leukemia, acute myelomonocytic leukemia, acute monocyte leukemia, B cell lines (JY, MST, BR17), EBV-infected B cell cultures, myeloid cell lines (U937, HL60, THP 1-0).

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: BALB/c mice were immunised with cells from acute monocytic leukemia patients.

Isotype: IgG kappa

Application Details

Application Notes: E11 fluorescence staining of normal blood cells was detectable on 90 % of monocytes, 95 % of neutrophils, and 14 %-24 % of lymphocytes (Hogg et al, 1984). Assay of E11 fluorescence-stained monocytes and neutrophils by EC3b rosette formation indicated that nearly all of the E11-stained cells formed rosettes with EC3b (Hogg et al, 1984). Treatment of erythrocytes, monocytes, neutrophils, and lymphocytes with E11 did not result in any detectable loss of CR1 activity as measured by EC3b rosette formation (Hogg et al, 1984). Immunofluorescence was used to determine E11 reactivity to a variety of cells, tissue sections, leukemias, and cell lines (Hogg et al, 1984). E11 has been used in ELISA experiments to quantify the amount of recombinant CR1 proteins (Park et al, 2013).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CR1

Background: CD35, Type 1 complement receptor, C3b/C4b receptor

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity